Scientist

Job Title: Scientist

Length of Contract: 12months

Location/Site: Worcester, MA ; 01604- 100% Onsite

What are the top 3-5 skills, experience or education required for this position:

1. Mammalian cell culture

2. Cell line engineering

3. Flow cytometry

4. Cell-based assays

5. Gene editing by CRISPR/Cas9

3-5 years of experience. Prefer someone who has worked at the Client or has 3-5 years of industry experience.

This opportunity is in the Cell Line Engineering group within Biotherapeutics Discovery which plays a key role in enabling reagent generation supporting diverse applications throughout Client's therapeutic development process. Under supervision of a senior scientist in the group, the individual will primarily support mammalian cell culture work, cell line engineering, including KO, KI and OE cell lines development, cellular assays, as well as assist with lab and inventory management and sample digitization initiatives. Opportunities to expand proficiencies in cell and molecular biology applications, such as cell and genome engineering, vector design, technology development, cell-based assays development, receptor density quantification, flow cytometry, cell sorting and cloning, and other cell line development technologies also exist.

Key responsibilities include:

* Mammalian cell culture (immortalized, cancer and primary lines)

* Generation and maintenance of cell-lines for antibody discovery and assay development.

* Cellular assays

* FACS analysis and sorting

* Technology development for cell line engineering

* Consolidation of historical and recent cell line assets into digital inventory solution.

* Expansion and banking of core cell lines to ensure adequate inventories.

* Coordination of cell line inventory stocks across multiple Client sites, providing a foundation for Cell Bank establishment.