Senior Research Technician RNA Synthesis

Mass General Brigham (Enterprise Services)
United States, Massachusetts, Cambridge
65 Landsdowne Street (Show on map)
May 02, 2025
GENERAL SUMMARY / OVERVIEW: The Gene and Cell Therapy Institute (GCTI) is a newly created system-wide Institute under Mass General Brigham. The Institute will foster a world-class gene and cell therapy program that will leverage ongoing, groundbreaking scientific research efforts across the MGB system. The mission of GCTI is to bring together the community of MGB investigators which can utilize gene and cell therapy platforms to achieve their scientific and clinical goals and improve the health of patients. The Institute will differentiate itself by harnessing the exceptional strengths of clinician scientists, the large and unique patient population, and major discoveries throughout the hospital system to propel MGB as the leader in Gene & Cell therapies. We are a motivated and technically proficient Senior Research Technician to join our RNA Therapeutics Core (RTC) team and participate in the development of novel RNA-based medicines and technologies. With guidance from senior scientific staff, the candidate who fills this role will develop RNA synthesis, purification, and analytical methods, and produce high quality RNA preparations for experimental applications and external parties. In addition to bench responsibilities, the individual will assist in day-to-day lab operations, including inventory management, equipment maintenance, and implementation of safety protocols. The successful candidate will have experience working in a research laboratory environment and be proficient in standard molecular biology techniques such as cloning, PCR, and electrophoresis. They would preferably have hands-on RNA synthesis experience, be detail-oriented and organized, and eager to work in a team environment. QUALIFICATIONS: Bachelor's degree in life sciences, biochemistry, or chemistry with at least 3 years of experience in a laboratory setting Good organizational skills, detail oriented Reproducibility of work with accuracy Sound degree of computer literacy Ability to methodically record data Good interpersonal and communication skills Ability to demonstrate professionalism Dedication to excellence PREFERRED COMPETENCIES: Experience working with mRNA, circular RNA, or long-noncoding RNA Experience with in vitro transcription and purification processes Experience with RNA process development Experience with lab management practice PRINCIPAL DUTIES AND RESPONSIBILITIES: Perform a variety of routine procedures following established methodologies including in vitro transcription, silica purification, tangential flow filtration, HPLC, cloning, gel electrophoresis, plasmid outgrowth and purification Perform analytical techniques and record quality metrics, with a strong focus on accuracy and reproducibility in work With supervision, develop new processes for RNA synthesis and purification, and implement these processes on automation platforms Operate laboratory equipment, instruments and robotics to perform repetitive laboratory techniques; learn how to use new instruments Use universal safety precautionsand proper personal protective equipment to protect self and co-workers from biohazardous materials; complete requisite trainings as appropriate Develop, implement, and enforce laboratory safety protocols in accordance with institutional and regulatory requirements (e.g., OSHA, IBC, EH&S). Coordinate lab staff training in biosafety, chemical hygiene, and emergency procedures. Serve as the liaison with Environmental Health & Safety (EH&S) and Biosafety Offices for inspections, compliance documentation, and hazard mitigation. Oversee and maintain accurate inventory of reagents and lab supplies. Coordinate ordering, shipping, receiving, and documentation of laboratory supplies and capital equipment Participate in lab meetings and generate ideas for improving efficiency and productivity in the lab's operations Communicate effectively with the team, discuss progress, and share data with supervisors All other duties as assigned Mass General Brigham Incorporated is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.