Research Affiliate I (Post Doctoral)

Compensation Grade:

Compensation Details:

Department

Job Description:

Responsibilities

The Research Affiliate I will work with a Principal Investigator to develop their post-doctoral skills set. The incumbent will be responsible for investigating the unexpected complexities of quorum sensing (QS) regulation in Pseudomonas aeruginosa and other Gram-negative pathogens. This project explores how protein-protein interactions influence promoter selectivity and transcriptional regulation by LuxR-type QS receptors-a paradigm-shifting concept in bacterial signaling networks. The incumbent will employ interdisciplinary techniques spanning molecular microbiology, proteomics, genomics, biochemistry, and structural biology to dissect the mechanisms by which accessory proteins control LuxR-type receptor activity and promoter binding specificity. For more information on the model system, please visit the lab website: https://www.wadsworth.org/research/laboratories/paczkowski-laboratory/paczkowski-laboratory-publications.

The Research Affiliate I will work with a Principal Investigator to develop their post-doctoral skills set which includes:

• Performing ChIP-seq and RNA-seq to map global gene regulation by LuxR-type transcription factors in Pseudomonas aeruginosa, Acinetobacter baumannii, Aeromonas hydrophila, and Chromobacterium violaceum.

• Generating and validating receptor-specific antibodies.

• Identifying and characterizing novel receptor-accessory protein interactions using mutagenesis screens (e.g., transposon or CRISPRi), FACS-based assays, and protein interaction studies (co-IP, bacterial 2-hybrid); integrate computational tools (e.g., AlphaFold Multimer) to model protein-protein interactions.

• Designing and testing mutant variants to probe the mechanistic basis of promoter selectivity.

• Contributing to manuscript writing, conference presentations, and other documents.

This position will be part of a dynamic team serving the Wadsworth Center's mission in the New York State Department of Health's efforts to protect and promote the health of New York's citizens. Come be a part of Science in the Pursuit of Health!

Minimum Qualifications

PhD in Biochemistry or Chemistry.

Preferred Qualifications

Experience with organic chemistry and chemical syntheses; experience designing or generating antibodies; experience authoring scientific manuscripts and a strong publication record.

Conditions of Employment

Grant funded position. Compliance with funding requirements such as time and effort reporting, grant deliverables, and contract deliverables, is required.

The selected candidate must reside within a reasonable commuting distance of the official work location specified in the job posting and must also be located in or willing to relocate to one of the following states: New York, New Jersey, Connecticut, Vermont, or Massachusetts, prior to hire. Telecommuting will not be available.

HRI participates in the E-Verify Program.

Affirmative Action/Equal Opportunity Employer/Qualified Individuals with Disabilities/Qualified Protected Veterans

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About Health Research, Inc.

Join us in our mission to make a difference in public health and advance scientific research! At Health Research, Inc. (HRI), your work will contribute to meaningful change and innovation in the communities we serve! At HRI, we are on a mission to transform the health and well-being of the people of New York State through innovative partnerships and cutting-edge public health initiatives. As a dynamic non-profit organization, HRIplays a crucial role in advancing the strategic goals of the New York State Department of Health (DOH), Roswell Park Comprehensive Cancer Center (RPCCC), and other health-related entities.